Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| leptin receptor | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Leptin Receptor recombinant protein (Position: R465-D839). Human Leptin Receptor shares 81% and 82% amino acid (aa) sequence identity with mouse and rat Leptin Receptor, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
LEPR |
Protein |
Leptin receptor |
Uniprot ID |
P48357 |
Function |
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity). |
Tissue Specificity |
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. |
Sub-cellular localization |
Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the type I cytokine receptor family. Type 2 subfamily. |
Aliases |
CD 295 antibody|CD295 antibody|CD295 antigen antibody|Db antibody|Fa antibody|HuB219 antibody|LEP R antibody|LEP-R antibody|LEPR antibody|LEPR_HUMAN antibody|LEPRD antibody|LEPROT antibody|Leptin receptor antibody|Leptin receptor fatty antibody|Leptin receptor gene related protein antibody|Leptin receptor precursor antibody|OB R antibody|OB R gene related protein antibody|OB receptor antibody|OB-R antibody|OB-RGRP antibody|obl antibody|Obr antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Leptin Receptor antibody, ASA-B1165–1.jpg
All lanes: Anti-Leptin Receptor(ASA-B1165) at 0.5ug/ml
WB: Recombinant Human Leptin Receptor Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti-Leptin Receptor(ASA-B1165) at 0.5ug/ml
WB: Recombinant Human Leptin Receptor Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
