Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nuclear receptor subfamily 1, group H, member 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human LXR alpha(371-385aa NVQDQLQVERLQHTY), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NR1H3 |
Protein |
Oxysterols receptor LXR-alpha |
Uniprot ID |
Q13133 |
Function |
Nuclear receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Exhibits a ligand-dependent transcriptional activation activity (PubMed:25661920). |
Tissue Specificity |
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the nuclear hormone receptor family. NR1 subfamily. |
Aliases |
Liver X receptor alpha antibody|LXR a antibody|LXRA antibody|NR1H3 antibody|NR1H3_HUMAN antibody|Nuclear orphan receptor LXR alpha antibody|Nuclear receptor subfamily 1 group H member 3 antibody|Oxysterols receptor LXR alpha antibody|Oxysterols receptor LXR-alpha antibody|RLD 1 antibody|RLD1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NR1H3 antibody, ASA-B1196, Western blotting
All lanes: Anti NR1H3 (ASA-B1196) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 51KD
Observed bind size: 51KD
All lanes: Anti NR1H3 (ASA-B1196) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 51KD
Observed bind size: 51KD
