Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| methyl-CpG binding domain protein 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4(566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MBD4 |
Protein |
Methyl-CpG-binding domain protein 4 |
Uniprot ID |
O95243 |
Function |
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 MBD (methyl-CpG-binding) domain. |
Aliases |
3 N(4) ethenocytosine glycosylase antibody|G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody|G/T mismatch glycosylase antibody|G/U mismatch glycosylase antibody|MBD 4 antibody|MBD4 antibody|MBD4_HUMAN antibody|MED 1 antibody|MED1 antibody|Methyl CpG binding domain protein 4 antibody|Methyl CpG binding endonuclease 1 antibody|Methyl CpG binding protein MBD4 antibody|Methyl-CpG-binding domain protein 4 antibody|Methyl-CpG-binding endonuclease 1 antibody|Methyl-CpG-binding protein MBD4 antibody|Mismatch specific DNA N glycosylase antibody|Mismatch-specific DNA N-glycosylase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MBD4 antibody, ASA-B1227, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD4, 39.7KD(162aa tag+ Q400-S580)
Lane 1: Recombinant Human MBD4 Protein 10ng
Lane 2: Recombinant Human MBD4 Protein 5ng
Lane 3: Recombinant Human MBD4 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD4, 39.7KD(162aa tag+ Q400-S580)
Lane 1: Recombinant Human MBD4 Protein 10ng
Lane 2: Recombinant Human MBD4 Protein 5ng
Lane 3: Recombinant Human MBD4 Protein 2.5ng
Anti-MBD4 antibody, ASA-B1227, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
