Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| chemokine (C-C motif) ligand 13 | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human MCP4 recombinant protein (Position: Q24-T98). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCL13 |
Protein |
C-C motif chemokine 13 |
Uniprot ID |
Q99616 |
Function |
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. |
Tissue Specificity |
Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the intercrine beta (chemokine CC) family. |
Aliases |
C-C motif chemokine 13 antibody|CCL13 antibody|CCL13_HUMAN antibody|Chemokine (C C motif) ligand 13 antibody|CK beta 10 antibody|CK-beta-10 antibody|CKb10 antibody|MCP-4 antibody|MGC17134 antibody|Monocyte chemoattractant protein 4 antibody|Monocyte chemotactic protein 4 antibody|NCC 1 antibody|NCC-1 antibody|NCC1 antibody|New CC chemokine 1 antibody|SCYA13 antibody|SCYL1 antibody|Small inducible cytokine A13 precursor antibody|Small inducible cytokine subfamily A (Cys Cys) member 13 antibody|Small-inducible cytokine A13 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MCP4 antibody, ASA-B1244–1.jpg
All lanes: Anti-MCP4(ASA-B1244) at 0.5ug/ml
WB: Recombinant Human MCP4 Protein 0.5ng
Predicted bind size: 34KD
Observed bind size: 34KD
All lanes: Anti-MCP4(ASA-B1244) at 0.5ug/ml
WB: Recombinant Human MCP4 Protein 0.5ng
Predicted bind size: 34KD
Observed bind size: 34KD
