Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| mitogen-activated protein kinase kinase 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MEK3(42-58aa RNLDSRTFITIGDRNFE), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MAP2K3 |
Protein |
Dual specificity mitogen-activated protein kinase kinase 3(MAP kinase kinase 3/MAPKK 3) |
Uniprot ID |
P46734 |
Function |
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
Tissue Specificity |
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. |
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. |
Aliases |
AW212142 antibody|Dual specificity mitogen activated protein kinase kinase 3 antibody|Dual specificity mitogen-activated protein kinase kinase 3 antibody|ERK kinase 3 antibody|MAP kinase kinase 3 antibody|MAP2K 3 antibody|map2k3 antibody|MAPK ERK kinase 3 antibody|MAPK kinase 3 antibody|MAPK/ERK kinase 3 antibody|MAPKK 3 antibody|MAPKK3 antibody|MEK 3 antibody|MEK3 antibody|Mitogen activated protein kinase kinase 3 antibody|MKK 3 antibody|MKK3 antibody|mMKK 3b antibody|mMKK3b antibody|MP2K3_HUMAN antibody|MPK 3 antibody|PRKMK 3 antibody|PRKMK3 antibody|Protein kinase mitogen activated kinase 3 antibody|protein kinase, mitogen-activated, kinase 3 antibody|SAPK kinase 2 antibody|SAPKK 2 antibody|SAPKK2 antibody|SKK2 antibody|Stress activated protein kinase kinase 2 antibody|zMKK 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEK3 antibody, ASA-B1254, All Western blotting
All lanes: Anti-MAP2K3(ASA-B1254) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: Rat Spleen Tissue Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: CEM Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti-MAP2K3(ASA-B1254) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: Rat Spleen Tissue Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: CEM Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
