Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| protein phosphatase 1, regulatory subunit 12A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Myosin Phosphatase(1-17aa MKMADAKQKRNEQLKRW), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PPP1R12A |
Protein |
Protein phosphatase 1 regulatory subunit 12A |
Uniprot ID |
O14974 |
Function |
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. |
Tissue Specificity |
Expressed in striated muscles, specifically in type 2a fibers (at protein level). |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Contains 6 ANK repeats. |
Aliases |
M130 antibody|MBS antibody|MGC133042 antibody|Myosin binding subunit antibody|Myosin phosphatase target subunit 1 antibody|Myosin phosphatase targeting subunit 1 antibody|Myosin phosphatase-targeting subunit 1 antibody|MYPT 1 antibody|MYPT1 antibody|MYPT1_HUMAN antibody|PPP1R12A antibody|Protein phosphatase 1 regulatory inhibitor subunit 12A antibody|Protein phosphatase 1 regulatory subunit 12A antibody|Protein phosphatase 1, regulatory(inhibitor) subunit 12A antibody|Protein phosphatase myosin binding subunit antibody|Protein phosphatase myosin-binding subunit antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Myosin Phosphatase antibody, ASA-B1341, Western blotting
All lanes: Anti Myosin Phosphatase (ASA-B1341) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD
All lanes: Anti Myosin Phosphatase (ASA-B1341) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD
