Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| nebulin |
Monoclonal |
IgG |
Mouse |
Chicken |
WB |
Ascites |
Immunogen
|
| Chicken breast nebulin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
NEB |
Protein
|
Nebulin |
Uniprot ID
|
Human(P20929) |
Function
|
This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin. |
Tissue Specificity
|
Muscle specific. Located in the thin filament of striated muscle. |
Sub-cellular localization
|
Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton. |
Sequence Similarities
|
Contains 178 nebulin repeats. |
Aliases
|
NEM2|NEB177D |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
1-2 μg/ml |
Chicken |
AssaySolutio’s ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
