Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| cholinergic receptor, nicotinic, alpha 1(muscle) |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1(22-36aa EHETRLVAKLFKDYS), identical to the related rat sequence, different from the related mouse sequence by one amino acid. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
CHRNA1 |
Protein
|
Acetylcholine receptor subunit alpha |
Uniprot ID
|
P02708 |
Function
|
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. |
Tissue Specificity
|
Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus. |
Sub-cellular localization
|
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
Sequence Similarities
|
|
Aliases
|
Acetylcholine receptor subunit alpha antibody|ACHA_HUMAN antibody|ACHRA antibody|ACHRD antibody|CHNRA antibody|Cholinergic receptor nicotinic alpha polypeptide 1 antibody|Cholinergic receptor, nicotinic, alpha polypeptide 1(muscle) antibody|CHRNA antibody|Chrna1 antibody|CMS2A antibody|FCCMS antibody|Nicotinic cholinergic receptor alpha 1 antibody|SCCMS antibody|Schizophrenia neurophysiologic defect candidate antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Rat Mouse |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody, ASA-B1379, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
