Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| NK2 homeobox 5 | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5 (103-132aa AKDPRAEKKELCALQKAVELEKTEADNAER), different from the related mouse and rat sequences by five amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NKX2-5 |
Protein |
Homeobox protein Nkx-2.5 |
Uniprot ID |
P52952 |
Function |
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. |
Tissue Specificity |
Expressed only in the heart. |
Sub-cellular localization |
Nucleus . |
Sequence Similarities |
Belongs to the NK-2 homeobox family. |
Aliases |
Cardiac-specific homeobox 1 antibody|Cardiac-specific homeobox antibody|CHNG5 antibody|CSX antibody|CSX1 antibody|FLJ52202 antibody|FLJ97166 antibody|FLJ97195 antibody|FLJ97197 antibody|FLJ99536 antibody|HLHS2 antibody|Homeobox protein CSX antibody|Homeobox protein NK-2 homolog E antibody|Homeobox protein Nkx 2.5 antibody|NK2 homeobox 5 antibody|NK2 transcription factor related locus 5 antibody|NK2 transcription factor related, locus 5 (Drosophila) antibody|Nkx2-5 antibody|NKX2.5 antibody|Nkx2E antibody|NKX4-1 antibody|Tinman paralog antibody|VSD3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NKX2 antibody, ASA-B1382, Western blotting
All lanes: Anti NKX2 (ASA-B1382) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti NKX2 (ASA-B1382) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
