Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| NADPH oxidase activator 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human NOXA1(176-195aa RQVPRGEVFRPHRWHLKHLE). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NOXA1 |
Protein |
NADPH oxidase activator 1 |
Uniprot ID |
Q86UR1 |
Function |
Functions as an activator of NOX1, a superoxide- producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. |
Tissue Specificity |
Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. |
Sub-cellular localization |
Cytoplasm. Cell membrane. Note: Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1. |
Sequence Similarities |
Belongs to the NCF2/NOXA1 family. |
Aliases |
Antigen NY CO 31 antibody|Antigen NY-CO-31 antibody|FLJ25475 antibody|Inhibitory NADPH oxidase activator 1 antibody|MGC131800 antibody|NADPH oxidase activator 1 antibody|NCF2 like protein antibody|NCF2-like protein antibody|NOX activator 1 antibody|NOXA 1 antibody|Noxa1 antibody|NOXA1_HUMAN antibody|NY CO 31 antibody|p51 nox antibody|p51-nox antibody|p51NOX antibody|P67phox like factor antibody|P67phox-like factor antibody|SDCCAG31 antibody|Serologically defined colon cancer antigen 31 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NOXA1 antibody, ASA-B1404, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
