Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nucleophosmin/nucleoplasmin 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human NPM2(25-43aa QERRTWTFRPQLEGKQSCR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NPM2 |
Protein |
Nucleoplasmin-2 |
Uniprot ID |
Q86SE8 |
Function |
Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus . Note: Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm. |
Sequence Similarities |
|
Aliases |
NPM2 antibody|NPM2_HUMAN antibody|nucleophosmin/nucleoplasmin, 2 antibody|nucleoplasmin 2 antibody|Nucleoplasmin-2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NPM2 antibody, ASA-B1407, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: SMMC Cell Lysate
