Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| ORAI calcium release-activated calcium modulator 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Orai1(278-301aa EFARLQDQLDHRGDHPLTPGSHYA), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ORAI1 |
Protein |
Calcium release-activated calcium channel protein 1 |
Uniprot ID |
Q96D31 |
Function |
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
Belongs to the Orai family. |
Aliases |
Calcium release activated calcium channel protein 1 antibody|Calcium release activated calcium modulator 1 antibody|Calcium release-activated calcium channel protein 1 antibody|CRACM 1 antibody|CRACM1 antibody|CRCM1 antibody|CRCM1_HUMAN antibody|FLJ14466 antibody|Orai 1 antibody|ORAI calcium release activated calcium modulator 1 antibody|orai1 antibody|ORAT1 antibody|Protein orai 1 antibody|Protein orai-1 antibody|TMEM 142A antibody|TMEM142A antibody|Transmembrane protein 142A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Orai1 antibody, ASA-B1434, Western blotting
All lanes: Anti Orai1 (ASA-B1434) at 0.5ug/ml
WB: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 50KD
All lanes: Anti Orai1 (ASA-B1434) at 0.5ug/ml
WB: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 50KD
