Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| cyclin-dependent kinase inhibitor 1B(p27, Kip1) |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rat |
ICC, WB |
Ascites |
Immunogen
|
| recombinant rodent p27Kip1 protein. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
CDKN1B |
Protein
|
Cyclin-dependent kinase inhibitor 1B |
Uniprot ID
|
Human(P46527), Mouse(P46414), Rat(O08769) |
Function
|
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
Tissue Specificity
|
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. |
Sub-cellular localization
|
Nucleus. Cytoplasm. Endosome . Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). |
Sequence Similarities
|
Belongs to the CDI family. |
Aliases
|
KIP1|MEN4|CDKN4|MEN1B|P27KIP1|Cyclin-dependent kinase inhibitor p27 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
2-4μg/ml |
Human, mouse, rat |
AssaySolutio’s ECL kit |
| Immunocytochemistry |
Suitable |
Human, mouse, rat |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
