Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| paxillin | Monoclonal | IgG | Mouse | Chicken, Human, Mouse, Rat | ICC, WB | Ascites |
Immunogen |
||||||
| C-terminal part of recombinant chicken paxillin(amino acids 305-559). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration |
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PXN |
Protein |
Paxillin |
Uniprot ID |
Human(P49023), Mouse(Q8VI36), Rat(Q66H76) |
Function |
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytoskeleton. |
Sequence Similarities |
Belongs to the paxillin family. |
Aliases |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 1-2μg/ml | Human, mouse, rat, chicken | AssaySolutio’s ECL kit |
| Immunocytochemistry | Suitable | Human, mouse, rat, chicken | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
-ASA-B1481-1.jpg)
Anti-Paxillin antibody (monoclonal), ASA-B1481, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate
-ASA-B1481-2.jpg)




