Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| programmed cell death 1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human PD1 recombinant protein (Position: P101-L288). Human PD1 shares 59% amino acid (aa) sequence identity with mouse PD1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PDCD1 |
Protein |
Programmed cell death protein 1 |
Uniprot ID |
Q15116 |
Function |
Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
|
Aliases |
CD279 antibody|CD279 antigen antibody|hPD l antibody|hPD-1 antibody|hSLE1 antibody|PD 1 antibody|PD-1 antibody|PD1 antibody|PDCD 1 antibody|PDCD1 antibody|PDCD1_HUMAN antibody|Programmed cell death 1 antibody|Programmed cell death protein 1 antibody|Protein PD 1 antibody|Protein PD-1 antibody|SLEB2 antibody|Systemic lupus erythematosus susceptibility 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PD1 antibody , ASA-B1492–1.jpg
All lanes: Anti PD1 (ASA-B1492) at 0.5ug/ml
WB: Recombinant Human PD1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti PD1 (ASA-B1492) at 0.5ug/ml
WB: Recombinant Human PD1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-PD1 antibody , ASA-B1492–2.jpg
All lanes: Anti PD1 (ASA-B1492) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 32KD
Observed bind size: 38KD
All lanes: Anti PD1 (ASA-B1492) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 32KD
Observed bind size: 38KD
