Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| phospholipase A2, group IVA(cytosolic, calcium-dependent) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Phospholipase A2(686-700aa PNQAFKRLHDLMHFN), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PLA2G4A |
Protein |
Cytosolic phospholipase A2 |
Uniprot ID |
P47712 |
Function |
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. |
Tissue Specificity |
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. |
Sub-cellular localization |
Cytoplasm. Cytoplasmic vesicle. Note: Translocates to membrane vesicles in a calcium-dependent fashion. |
Sequence Similarities |
Contains 1 C2 domain. |
Aliases |
Calcium dependent phospholipid binding protein antibody|CPLA 2 antibody|cPLA2 alpha antibody|cPLA2 antibody|Cytosolic phospholipase A2 antibody|Cytosolic phospholipase A2 group IVA antibody|Lysophospholipase antibody|MGC126350 antibody|PA24A_HUMAN antibody|Phosphatidylcholine 2 acylhydrolase antibody|Phosphatidylcholine 2-acylhydrolase antibody|Phospholipase A2 group 4 A antibody|Phospholipase A2 group IVA(cytosolic calcium dependent) antibody|Phospholipase A2 group IVA antibody|PhospholipaseA2 antibody|PLA2G4 antibody|pla2g4a antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Phospholipase A2 antibody, ASA-B1517, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: COLO320 Cell Lysate
