Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| RAB3C, member RAS oncogene family | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3C(204-217aa AITAAKQNTRLKET), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
RAB3C |
Protein |
Ras-related protein Rab-3C |
Uniprot ID |
Q96E17 |
Function |
Protein transport. Probably involved in vesicular traffic (By similarity). |
Tissue Specificity |
Expressed in brain, placenta and lung. |
Sub-cellular localization |
Cell membrane ; Lipid-anchor ; Cytoplasmic side . |
Sequence Similarities |
|
Aliases |
RAB3C member RAS oncogene family antibody|Ras related protein Rab3C antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Rab3C antibody, ASA-B1600, All Western blotting
All lanes: Anti-RAB3C(ASA-B1600) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
All lanes: Anti-RAB3C(ASA-B1600) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
