Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| retinol binding protein 4, plasma | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human RBP4 recombinant protein (Position: E19-201L). Human RBP4 shares 86% amino acid (aa) sequence identity with both mouse and rat RBP4. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
RBP4 |
Protein |
Retinol-binding protein 4 |
Uniprot ID |
P02753 |
Function |
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the calycin superfamily. Lipocalin family. |
Aliases |
OTTHUMP00000020114 antibody|OTTHUMP00000020115 antibody|OTTHUMP00000020116 antibody|Plasma retinol binding protein 4 antibody|Plasma retinol-binding protein antibody|Plasma retinol-binding protein(1-176) antibody|PRBP antibody|PRO2222 antibody|RBP antibody|RBP4 antibody|RDCCAS antibody|RET4_HUMAN antibody|Retinol binding protein 4 antibody|Retinol binding protein 4 interstitial antibody|Retinol binding protein 4 plasma antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-RBP4 antibody, ASA-B1617, Western blotting
All lanes: Anti RBP4 (ASA-B1617) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti RBP4 (ASA-B1617) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
