Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| snail family zinc finger 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human SNAIL(127-155aa AAFPGLGQVPKQLAQLSEAKDLQARKAFN), different from the related mouse sequence by six amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SNAI1 |
Protein |
Zinc finger protein SNAI1 |
Uniprot ID |
O95863 |
Function |
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5′-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. |
Tissue Specificity |
Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. |
Sub-cellular localization |
Nucleus. Cytoplasm. Note: Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. |
Sequence Similarities |
Belongs to the snail C2H2-type zinc-finger protein family. |
Aliases |
dJ710H13.1 antibody|Protein sna antibody|Protein snail homolog 1 antibody|Protein snail homolog antibody|SLUGH2 antibody|SNA antibody|Sna protein antibody|SNAH antibody|SNAI antibody|SNAI1 antibody|SNAI1_HUMAN antibody|Snail 1 homolog antibody|Snail 1 zinc finger protein antibody|SNAIL antibody|Snail homolog 1 (Drosophila) antibody|SNAIL, Drosophila, homolog of, 1 antibody|SNAIL1 antibody|Zinc finger protein SNAI1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SNAIL antibody, ASA-B1738, Western blotting
All lanes: Anti SNAIL (ASA-B1738) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti SNAIL (ASA-B1738) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 39KD
Observed bind size: 39KD
