Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| suppressor of cytokine signaling 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SOCS2(181-198aa LPLPTRLKDYLEEYKFQV), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SOCS2 |
Protein |
Suppressor of cytokine signaling 2(SOCS-2) |
Uniprot ID |
O14508 |
Function |
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
Tissue Specificity |
High expression in heart, placenta, lung, kidney and prostate. |
Sub-cellular localization |
|
Sequence Similarities |
Contains 1 SH2 domain. |
Aliases |
CIS 2 antibody|CIS-2 antibody|CIS2 antibody|Cish 2 antibody|Cish2 antibody|Cytokine inducible SH2 protein 2 antibody|Cytokine-inducible SH2 protein 2 antibody|SOCS 2 antibody|SOCS-2 antibody|Socs2 antibody|SOCS2_HUMAN antibody|SSI 2 antibody|SSI-2 antibody|SSI2 antibody|STAT induced STAT inhibitor 2 antibody|STAT-induced STAT inhibitor 2 antibody|STATI 2 antibody|STATI2 antibody|Suppressor of cytokine signaling 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOCS2 antibody, ASA-B1745, Western blotting
All lanes: Anti SOCS2 (ASA-B1745) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HT1080 Tissue Lysate at 50ug
Lane 7: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 8: Mouse Liver Tissue Lysate at 50ug
Lane 9: Mouse Spleen Tissue Lysate a
All lanes: Anti SOCS2 (ASA-B1745) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HT1080 Tissue Lysate at 50ug
Lane 7: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 8: Mouse Liver Tissue Lysate at 50ug
Lane 9: Mouse Spleen Tissue Lysate a
