Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| Sp4 transcription factor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SP4 (592-620aa AVSPDQLTQVHLQQGQQTSDQEVQPGKRL), different from the related mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SP4 |
Protein |
Transcription factor Sp4 |
Uniprot ID |
Q02446 |
Function |
Binds to GT and GC boxes promoters elements. Probable transcriptional activator. |
Tissue Specificity |
Abundant in brain. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the Sp1 C2H2-type zinc-finger protein family. |
Aliases |
HF1B antibody|MGC130008 antibody|MGC130009 antibody|Sp4 antibody| SP4_ HUMAN antibody|SPR-1 antibody|SPR1 antibody|Transcription factor Sp4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SP4 antibody, ASA-B1768, Western blotting
All lanes: Anti SP4 (ASA-B1768) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
All lanes: Anti SP4 (ASA-B1768) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD
