Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| tafazzin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Tafazzin(162-178aa MDFILEKLNHGDWVHIF), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TAZ |
Protein |
Tafazzin |
Uniprot ID |
Q16635 |
Function |
Some isoforms may be involved in cardiolipin (CL) metabolism. |
Tissue Specificity |
High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N- terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes. |
Sub-cellular localization |
Isoform 1: Membrane; Single-pass membrane protein. |
Sequence Similarities |
Belongs to the taffazin family. |
Aliases |
Barth syndrome antibody|Cardiomyopathy dilated 3A(X linked) antibody|EFE2 antibody|Endocardial fibroelastosis 2 antibody|Protein G4.5 antibody|Tafazzin antibody|TAZ antibody|TAZ protein antibody|TAZ_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Tafazzin/TAZ antibody, ASA-B1831, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: SCG Cell Lysate
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: SCG Cell Lysate
