Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| DNA nucleotidylexotransferase | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DNTT |
Protein |
DNA nucleotidylexotransferase |
Uniprot ID |
P04053 |
Function |
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5′-triphosphate to the 3′-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the DNA polymerase type-X family. |
Aliases |
Deoxynucleotidyltransferase terminal antibody|DNA nucleotidylexotransferase antibody|DNTT antibody|Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody|TDT antibody|TDT_HUMAN antibody|Terminal addition enzyme antibody|Terminal deoxynucleotidyltransferase antibody|Terminal deoxyribonucleotidyltransferase antibody|Terminal transferase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TdT antibody, ASA-B1841–1.jpg
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: Recombinant Human TDT Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: Recombinant Human TDT Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-TdT antibody, ASA-B1841–2.jpg
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
