Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| TIMP metallopeptidase inhibitor 4 | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP4(209-224aa RGHLPLRKEFVDIVQP). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TIMP4 |
Protein |
Metalloproteinase inhibitor 4 |
Uniprot ID |
Q99727 |
Function |
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. |
Tissue Specificity |
Abundant in heart and present at low levels in many other tissues. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the protease inhibitor I35 (TIMP) family. |
Aliases |
Metalloproteinase inhibitor 4 antibody|TIMP 4 antibody|TIMP metallopeptidase inhibitor 4 antibody|TIMP-4 antibody|Timp4 antibody|TIMP4_HUMAN antibody|Tissue inhibitor of metalloproteinase 4 antibody|Tissue inhibitor of metalloproteinases 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TIMP4 antibody, ASA-B1865, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 1: HT1080 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
