Description
Data Sheet
| Assay principle | The Novateinbio Human Complement C3d ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of complement C3d in human plasma, serum, milk, urine, saliva, CSF, cell culture, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human complement C3d in less than 5 hours. A polyclonal antibody specific for human complement C3d has been pre-coated onto a 96-well microplate with removable strips. Complement C3d in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human complement C3d, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. |
| Assay Range | 0.28 – 18 ng/ml |
| Assay Type | Sandwich ELISA |
| Background | The IgG antibody response creates sensitivity to a particular food. Symptoms may be less severe than with IgE allergic reaction and can manifest anywhere from 3-72 hours after exposure. IgG reactions create inflammation that makes many pathologies worse. The delayed response makes sensitivities difficult to identify without a diagnostic test. Sensitivities can improve with treatment and improved gut health. C3d is a complement antigen and an activator of our complement cascade system. Reaction to the specified food will worsen if C3d activation is present along with an IgG antibody response. The C3 protein attaches to the antigen and amplifies the IgG response, increasing the inflammatory potential of IgG titre. Complement is not dependent on exposure or antibody presence and represents innate immune function. IgG4, which is a subclass of IgG, is a distinct antibody in the immune system. IgG4 total antibody is important in relation to IgE because this antibody acts as a blocking agent for an IgE reaction. When the IgG4 reaction is greater than the IgE reaction for a particular antigen, IgG4 blocks the IgE antibodies from binding to the receptor sites and releasing histamine, thereby reducing severity of the symptoms associated with the IgE reaction. This is referred to as the blocking potential. IgG4 carries its own clinical relevance in high levels and may mediate several conditions and diseases. |
| Kit Type | Colorimetric |
| Sample Type | Serum, Plasma |
