Description
|
Assay Range |
78 – 5,000 pg/mL |
|
Sensitivity |
5.0 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Human CD25 standard: 2 vials
2. One 96-well plate coated with Human CD25 Ab
3. Sample diluent buffer: 12 mL – 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Interleukin-2 receptor subunit alpha (IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA), also known as TAC antigen, p55 or CD25, is a transmembrane glycoprotein that binds to IL-2 and transduce the downstream signals in IL-2 activated cells. The biological activities of IL-2 are mediated by its binding to a multi-molecular cellular receptor complex which is made up of 3 subunits – alpha (α), beta (β) and gamma (γ). The α and β chains are required for binding IL-2, while signal transduction following IL-2 activation is carried out by the γ-chain and the β subunit. IL-2R β and IL-2Rγ are members of the type I cytokine receptor family, whereas IL-2Rα is structurally related only to the IL-15 Rα chain.
A soluble form of IL-2Rα (s IL-2Rα) has been found in serum, concomitant with its increased expression on cells. The soluble form of IL-2Rα may be a poor inhibitor of IL-2 according to its low binding affinity. Increased levels of the soluble IL-2 Rα in biological fluids have been correlated with increased T and B cell activation and immune system activation.
