Description
Sensitivity
150 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human vWF standard: 10 ng/vial – 2.
2. One 96-well plate precoated with anti-human vWF Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin conjugated anti-Human vWF Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 60µL, dilution 1:200.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Von Willebrand factor (vWF) is a blood glycoprotein involved in hemostasis. It is deficient or defective in von Willebrand disease and is involved in a large number of other diseases, including thrombotic thrombocytopenic purpura, Heyde’s syndrome, and possibly hemolytic-uremic syndrome. Increased plasma levels in a large number of cardiovascular, neoplastic, and connective tissue diseases are presumed to arise from adverse changes to the endothelium, and may contribute to an increased risk of thrombosis.
