Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| angiopoietin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ANGPT2 |
Protein |
Angiopoietin-2 |
Uniprot ID |
O15123 |
Function |
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Contains 1 fibrinogen C-terminal domain. |
Aliases |
AGPT 2 antibody|Agpt2 antibody|ANG 2 antibody|ANG-2 antibody|ANG2 antibody|ANG2 antibody|Angiopoietin 2a antibody|Angiopoietin 2B antibody|Angiopoietin-2 antibody|Angiopoietin2 antibody|ANGP2_HUMAN antibody|ANGPT 2 antibody|ANGPT2 antibody|ANGPT2 antibody|Tie2 ligand antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Angiopoietin 2 antibody, ASA-B0085, IHC(P)
Human Placenta Tissue
Anti-Angiopoietin 2 antibody, ASA-B0085, IHC(P)
Rat Intestine Tissue
Anti-Angiopoietin 2 antibody, ASA-B0085, IHC(P)
Mouse Intestine Tissue
