TECHNICAL INFORMATION

TECHNICAL INFORMATION

• ELISA Troubleshooting Guide
• Chemiluminescence Immunoassay (CLIA)
• Competition ELISA Kit Instruction
• Converting protein mass concentration to molar concentration, or vice versa
• Direct and Competitive ELISAs Illustration
• Dot Blot Protocol
• Quantitative Elisa Kit Instruction
• Quantitative Elisa Kit Principle
• Streamlined Assay Result Calculation

• Western Blot Protocol

Loading Controls for Western Blot

Whole Cell: GAPDH, beta actin, alpha actin, alpha tubulin, beta tubulin, HSP90, HSC70, HSP70, cyclophilin B, cofilin

Cytoplasm: Alpha tubulin, beta tubulin, alpha actin, beta actin, cofilin

Nucleus: PCNA, lamin B1, TBP, HDAC1, YY1, histone H3

Membrane: Na/K ATPase

Mitochondria: COX IV, VDAC1/Porin, HSP60