Description
Assay Format
Sandwich ELISA.
Storage
Store at 4 °C.
Expiration
6 months at 4 °C.
Range
156 pg/mL – 10000 pg/mL.
Sensitivity
150 pg/mL.
Specificity
No detectable cross-reactivity with any other cytokine.
Application
cell culture supernatants, serum and plasma.
Kit Components
1. Lyophilized recombinant Human CD19 standard: 10 ng/tube-2.
2. One 96-well plate precoated with anti-Human CD19 antibody.
3. Sample diluent buffer: 10 mL – 1
4. Anti-Human CD17 detection antibody-1, dilution 1:2000.
5. Antibody diluent buffer: 10ml – 1.
6. Chromogenic Solution A: 6ml – 1.
7. Chromogenic Solution B: 6ml – 1.
8. Stop solution: 10ml – 1.
Background
B-lymphocyte antigen CD19, also known as CD19, is a single-pass type I membrane protein which was assembled with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. CD19 is an important differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. It interacts with VAV, GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. Defects in CD19 are a cause of hypogammaglobulinemia and results in common variable immunodeficiency.
