Description
Human C-Telopeptide Of Type I Callagen ELISA Kit
For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.
Data Sheet
| Assay principle | The Human CTX-1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Human CTX-1 specific antibody has been pre-coated onto 96-well plate. Human CTX-1 present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human CTX-1 detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human CTX-1 captured on plate. |
| Assay Range | 31.3 — 2000 pg/mL |
| Assay time | 4.5 hours |
| Storage | 2-8 °C |
