Description
Data Sheet
| Alternate Names | HAMP; HEPC; hepcidin antimicrobial peptide; Hepcidin; HEPCPutative liver tumor regressor; HFE2B; HFE2Bhepcidin; LEAP1; LEAP-1; LEAP1PLTR; Liver-expressed antimicrobial peptide 1; PLTR |
| Amino acid sequence | ICIFCCGCCHRSKCGMCCKT |
| Assay principle | The Human Hepcidin ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Human Hepcidin specific antibody has been pre-coated onto 96-well plate. Human Hepcidin present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human Hepcidin detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human Hepcidin captured on plate. |
| Assay Range | 12.5 – 800 pg/ml |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Dilution Factors | 5 to 60 folds for plasma and serum |
| Gene ID | 57817 |
| Kit Type | Colorimetric ELISA |
| Precision | Intra-Assay, 5.6%; Inter-Assay, 9.9% |
| Recovery | 94% to 113% |
| Sample Type | Plasma, Serum, Urine |
| Sample Volume | 100 ul |
| Sensitivity | 1.25 pg/ml |
| Size | 96T |
| Specificity | recognize both native and well folded recombinant human hepcidin |
| Storage | Storage of components varies. See protocol for specific instructions. |
