Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| ATP-binding cassette, sub-family A(ABC1), member 4 |
Polyclonal |
IgG |
Rabbit |
Human |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA4(1893-1906aa TLLVQRHFFLSQWI). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
ABCA4 |
Protein
|
Retinal-specific ATP-binding cassette transporter |
Uniprot ID
|
P78363 |
Function
|
In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans- retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery. |
Tissue Specificity
|
Retinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells. |
Sub-cellular localization
|
Membrane. |
Sequence Similarities
|
Belongs to the ABC transporter superfamily. ABCA family. |
Aliases
|
ABC 10 antibody|ABC A4 antibody|ABC10 antibody|ABCA 4 antibody|abcA4 antibody|ABCA4_HUMAN antibody|ABCR antibody|ARMD 2 antibody|ARMD2 antibody|ATP binding cassette 10 antibody|ATP binding cassette sub family A member 4 antibody|ATP binding cassette sub family A member4 antibody|ATP binding cassette transporter antibody|ATP binding cassette transporter retinal specific antibody|ATP binding cassette, sub family A(ABC1), member 4 antibody|ATP binding cassette, sub family A(ABC1), member4 antibody|ATP binding cassette10 antibody|ATP binding transporter, retina specific antibody|ATP-binding cassette sub-family A member 4 antibody|CORD 3 antibody|CORD3 antibody|DKFZp781N1972 antibody|FFM antibody|FLJ17534 antibody|Photoreceptor rim protein antibody|Retina specific ABC transporter antibody|Retinal specific ATP binding cassette transporter antibody|Retinal-specific ATP-binding cassette transporter antibody|RIM ABC transporter antibody|RIM protein antibody|RmP antibody|RP 19 antibody|RP19 antibody|Stargardt disease protein antibody|STGD antibody|STGD1 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ABCA4 antibody, ASA-B0013, Western blotting
WB: U87 Cell Lysate
