Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| actin, gamma 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Actin(365-375aa SGPSIVHRKCF), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Liquid |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ACTG1 |
Protein |
Actin, cytoplasmic 2 |
Uniprot ID |
P63261 |
Function |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytoskeleton. |
Sequence Similarities |
Belongs to the actin family. |
Aliases |
Gamma-actin |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Actin antibody, ASA-B0030, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: MM231 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: SW620 Cell Lysate
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: MM231 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: SW620 Cell Lysate
Anti-Actin antibody, ASA-B0030, IHC(P)
Rat Cardiac Muscle Tissue
Rat Cardiac Muscle Tissue
