Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| actin, alpha 1, skeletal muscl | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ACTA1 |
Protein |
Actin, alpha skeletal muscle |
Uniprot ID |
P68133 |
Function |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytoskeleton. |
Sequence Similarities |
|
Aliases |
ACTA antibody|ACTA1 antibody|Actin alpha skeletal muscle antibody|Actin antibody|actin, alpha 1, skeletal muscle 1 antibody|actin, alpha 1, skeletal muscle antibody|Actin, alpha skeletal muscle antibody|actina antibody|actine antibody| ACTS_HUMAN antibody|aktin antibody|Alpha Actin 1 antibody|Alpha skeletal muscle Actin antibody|alpha skeletal muscle antibody|alpha-actin antibody|Alpha-actin-1 antibody|ASMA antibody|CFTD antibody| CFTD1antibody|CFTDM antibody| MPFD antibody|NEM1 antibody|NEM2 antibody|NEM3 antibody| nemaline myopathy type 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ACTIN antibody, ASA-B0031, Western blotting
All lanes: Anti ACTIN (ASA-B0031) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted band size: 42KD
Observed band size: 42KD
All lanes: Anti ACTIN (ASA-B0031) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted band size: 42KD
Observed band size: 42KD
Anti- ACTIN antibody, ASA-B0031, IHC(P)
Mouse Intestine Tissue
Mouse Intestine Tissue
Anti- ACTIN antibody, ASA-B0031, IHC(P)
Rat Spleen Tissue
Rat Spleen Tissue
