Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| ADAM metallopeptidase domain 2 |
Polyclonal |
IgG |
Rabbit |
Human |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM2(701-715aa VLIAIMVKVNFQRKK). |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
ADAM2 |
Protein
|
Disintegrin and metalloproteinase domain-containing protein 2 |
Uniprot ID
|
Q99965 |
Function
|
Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. |
Tissue Specificity
|
Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues. |
Sub-cellular localization
|
Membrane; Single-pass type I membrane protein. |
Sequence Similarities
|
Contains 1 disintegrin domain. |
Aliases
|
A disintegrin and metalloproteinase domain 2 antibody|ADAM 2 antibody|ADAM metallopeptidase domain 2 antibody|Adam2 antibody|ADAM2_HUMAN antibody|AI323749 antibody|Cancer/testis antigen 15 antibody|CRYN1 antibody|CRYN2 antibody|CT15 antibody|Disintegrin and metalloproteinase domain-containing protein 2 antibody|Fertilin beta antibody|Fertilin subunit beta antibody|Ftnb antibody|PH 30 antibody|PH 30b antibody|PH-30 antibody|PH30 antibody|Ph30 beta antibody|PH30-beta antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADAM2 antibody, ASA-B0035, Western blotting
SMMC Cell Lysate
