Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| ADAM metallopeptidase with thrombospondin type 1 motif, 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS5(772-787aa TAYLALKKKNGEYLIN), different from the related mouse and rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ADAMTS5 |
Protein |
A disintegrin and metalloproteinase with thrombospondin motifs 5 |
Uniprot ID |
Q9UNA0 |
Function |
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. |
Tissue Specificity |
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. |
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix . |
Sequence Similarities |
Contains 1 disintegrin domain. |
Aliases |
A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody|A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody|A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 5 antibody|A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif, 5(aggrecanase 2) antibody|A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody|ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody|ADAM TS 11 antibody|ADAM TS 5 antibody|ADAM TS5 antibody|ADAM-TS 11 antibody|ADAM-TS 5 antibody|ADAM-TS5 antibody|ADAMTS 11 antibody|ADAMTS 5 antibody|ADAMTS-11 antibody|ADAMTS-5 antibody|ADAMTS11 antibody|Adamts5 antibody|ADMP 2 antibody|ADMP-2 antibody|ADMP2 antibody|Aggrecanase 2 antibody|Aggrecanase-2 antibody|ATS5_HUMAN antibody|FLJ36738 antibody|Implantin antibody|ThromboSpondin motif-5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ADAMTS5 antibody, ASA-B0039, IHC(P)
Rat Brain Tissue
Rat Brain Tissue
Anti-ADAMTS5 antibody, ASA-B0039, Western blotting
All lanes: Anti ADAMTS5 (ASA-B0039) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 102KD
Observed bind size: 102KD
All lanes: Anti ADAMTS5 (ASA-B0039) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 102KD
Observed bind size: 102KD
Anti-ADAMTS5 antibody, ASA-B0039, IHC(P)
Rat Brain Tissue
Rat Brain Tissue
