Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY |
| Description | AK4 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 243 amino acids (1-223a.a.) and having a molecular mass of 27.4kDa. AK4 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography. |
| Expression host | Escherichia Coli. |
| Formulation | AK4 Human solution (1mg/1ml) containing 20mM Tris-HCl pH-8, 2mM DTT, 0.1M NaCl, and 10% glycerol. |
| Protein Background | AK4 belongs to the adenylate kinase family of enzymes. AK4 protein is restricted to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide structures in a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Hereditary mutations which cause AK4 deficiencies in erythrocytes have been associated to hemolytic anemia. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | Adenylate Kinase 4, AK3, Adenylate Kinase 3-like 1, AK3L1, ATP-AMP transphosphorylase, Adenylate Kinase 3, GTP:AMP phosphotransferase, mitochondrial adenylate kinase-3, AK3L2, MGC166959, EC 2.7.4.3. |
