Description
Data Sheet
| Coating concentration | 0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination. |
| Description | Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. |
| Expression host | Sf9 insect cells. |
| Formulation | PL-12 is supplied in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test). |
| Protein Background | Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Synonyms | Alanyl-tRNA synthetase cytoplasmic, EC 6.1.1.7, Alanine-tRNA ligase, AlaRS, Renal carcinoma antigen NY-REN-42, PL-12, AARS. |
