Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| actinin, alpha 2 |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rabbit, Rat |
IHC-P, IHC-F, WB |
Ascites |
Immunogen
|
| Rabbit skeletal alpha-actinin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
ACTN2 |
Protein
|
Alpha-actinin-2 |
Uniprot ID
|
Human(P35609), Mouse(Q9JI91), Rat(D3ZCV0) |
Function
|
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
Tissue Specificity
|
Expressed in both skeletal and cardiac muscle. |
Sub-cellular localization
|
Cytoplasm, myofibril, sarcomere, Z line. |
Sequence Similarities
|
Belongs to the alpha-actinin family. |
Aliases
|
CMD1AA|Alpha-actinin skeletal muscle isoform 2|F-actin cross-linking protein |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
3.5μg/ml |
Human, mouse, rat, rabbit. |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
3.5-7μg/ml |
Human, mouse, rat, rabbit. |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
3.5-7μg/ml |
Human, mouse, rat, rabbit. |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
