Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| amylase, alpha 1A(salivary) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1(30-44aa HLFEWRWVDIALECE), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
AMY1A |
Protein |
Alpha-amylase 1 |
Uniprot ID |
P04745 |
Function |
|
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the glycosyl hydrolase 13 family. |
Aliases |
1 antibody|4-alpha-D-glucan glucanohydrolase 1 antibody|Alpha-amylase 1 antibody|AMY1_HUMAN antibody|AMY1A antibody|AMY1B antibody|AMY1C antibody|Salivary alpha-amylase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Alpha Amylase 1 antibody, ASA-B0075, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Anti-Alpha Amylase 1 antibody, ASA-B0075, IHC(P)
Rat Spleen Tissue
Rat Spleen Tissue
