Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| annexin A1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequences identity with mouse and rat Annexin A1, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ANXA1 |
Protein |
Annexin A1 |
Uniprot ID |
P04083 |
Function |
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus . Cytoplasm . Cell projection, cilium . Basolateral cell membrane . Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. |
Sequence Similarities |
Belongs to the annexin family. |
Aliases |
Annexin 1 antibody|Annexin A1 antibody|Annexin I (lipocortin I) antibody|Annexin I antibody|Annexin-1 antibody|AnnexinA1 antibody|AnnexinI antibody|ANX 1 antibody|ANX A1 antibody|ANX1 antibody|ANXA 1 antibody|ANXA1 antibody|ANXA1 protein antibody|ANXA1_HUMAN antibody|Calpactin 2 antibody|Calpactin II antibody|Calpactin-2 antibody|CalpactinII antibody|Chromobindin 9 antibody|Chromobindin-9 antibody|Chromobindin9 antibody|Lipocortin 1 antibody|Lipocortin I antibody|Lipocortin1 antibody|LipocortinI antibody|LPC 1 antibody|LPC1 antibody|p35 antibody|Phospholipase A2 inhibitory protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Annexin A1 antibody, ASA-B0093, Western blot
All lanes: Anti AnnexinA1 (ASA-B0093) at 0.5ug/ml
WB: Recombinant Human AnnexinA1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
WB: Recombinant Human AnnexinA1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Annexin A1 antibody, ASA-B0093, Western blot
All lanes: Anti AnnexinA1 (ASA-B0093) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A549 Whole Cell Lysate at 40ug
Lane 9: JURKAT Whole Cell Lysate at 40ug
All lanes: Anti AnnexinA1 (ASA-B0093) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A549 Whole Cell Lysate at 40ug
Lane 9: JURKAT Whole Cell Lysate at 40ug
Anti-Annexin A1 antibody, ASA-B0093, IHC(P)
Human Tonsil Tissue
Anti-Annexin A1 antibody, ASA-B0093, IHC(P)
Rat Lung Tissue
