Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| annexin A3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence in the middle region of human Annexin A3 (153-184aa RKALLTLADGRRDESLKVDEHLAKQDAQILYK), different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ANXA3 |
Protein |
Annexin A3 |
Uniprot ID |
P12429 |
Function |
Inhibitor of phospholipase A2, also possesses anti- coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. |
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the annexin family. |
Aliases |
2-cyclic phosphate 2-phosphohydrolase antibody|35-alpha calcimedin antibody| Annexin A3 antibody|Annexin III antibody|Annexin-3 antibody|ANX3 antibody|ANXA 3 antibody|Anxa3 antibody|ANXA3_HUMAN antibody|Inositol 1 2 cyclic phosphate 2 phosphohydrolase antibody| Inositol 1 antibody|Lipocortin III antibody| OTTHUMP00000160701 antibody| OTTHUMP00000219237 antibody| OTTHUMP00000219238 antibody|PAP III antibody|PAP-III antibody| Placental anticoagulant protein III antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Annexin A3 antibody, ASA-B0097, Western blotting
All lanes: Anti Annexin A3 (ASA-B0097) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Stomach Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted band size: 36KD
Observed band size: 36KD
All lanes: Anti Annexin A3 (ASA-B0097) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Stomach Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted band size: 36KD
Observed band size: 36KD
Anti- Annexin A3 antibody, ASA-B0097, IHC(P)
Mouse Cardiac Muscle Tissue
Mouse Cardiac Muscle Tissue
Anti- Annexin A3 antibody, ASA-B0097, IHC(P)
Rat Cardiac Muscle Tissue
Rat Cardiac Muscle Tissue
