Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nucleolar protein 3(apoptosis repressor with CARD doma) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD(91-106aa WDWQHVGPGYRDRSYD), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NOL3 |
Protein |
Nucleolar protein 3 |
Uniprot ID |
O60936 |
Function |
Isoform 1: May be involved in RNA splicing. |
Tissue Specificity |
Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas. |
Sub-cellular localization |
Isoform 1: Nucleus, nucleolus. |
Sequence Similarities |
Contains 1 CARD domain. |
Aliases |
Apoptosis repressor with CARD antibody|ARC antibody|Muscle enriched cytoplasmic protein antibody|Muscle-enriched cytoplasmic protein antibody|MYC antibody|MYP antibody|Nol3 antibody|NOL3_HUMAN antibody|NOP antibody|Nop30 antibody|Nucleolar protein 3(apoptosis repressor with CARD domain) antibody|Nucleolar protein 3 antibody|Nucleolar protein of 30 kDa antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Apoptosis repressor with CARD antibody, ASA-B0127, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: Rat Liver Tissue Lysate
Lane 1: SMMC Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: Rat Liver Tissue Lysate
