Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| aspartate beta-hydroxylase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ASPH (726-758aa, EVWQDASSFRLIFIVDVWHPELTP- QQRRSLPAI), identical to the related mouse sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ASPH |
Protein |
Aspartyl/asparaginyl beta-hydroxylase |
Uniprot ID |
Q12797 |
Function |
Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. |
Tissue Specificity |
Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level). |
Sub-cellular localization |
Isoform 1: Endoplasmic reticulum membrane; Single-pass type II membrane protein. |
Sequence Similarities |
Belongs to the aspartyl/asparaginyl beta-hydroxylase family. |
Aliases |
A beta H J J antibody|AAH antibody|ASP beta hydroxylase antibody| Aspartyl/ asparaginyl beta hydroxylase antibody|ASPH antibody|ASPH_HUMAN antibody| BAH antibody|Cardiac junctin antibody|CASQ2BP1 antibody|HAAH antibody| Humbug antibody|JCTN antibody|junctin antibody| Peptide aspartate beta dioxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ASPH antibody, ASA-B0158, Western blotting
All lanes: Anti ASPH (ASA-B0158) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted band size: 86KD
Observed band size: 100KD
All lanes: Anti ASPH (ASA-B0158) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted band size: 86KD
Observed band size: 100KD
Anti- ASPH antibody, ASA-B0158,IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue
