Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| activating transcription factor 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human ATF1 recombinant protein (Position: M1-V271). Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
19,261,910 |
Protein |
Cyclic AMP-dependent transcription factor ATF-1 |
Uniprot ID |
P18846 |
Function |
This protein binds the cAMP response element (CRE) (consensus: 5′-GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the bZIP family. ATF subfamily. |
Aliases |
Activating transcription factor 1 antibody|ATF 1 antibody|atf1 antibody|ATF1 EWS fusion gene antibody|ATF1 FUS fusion gene antibody|ATF1_HUMAN antibody|cAMP dependent transcription factor 1 antibody|cAMP-dependent transcription factor ATF-1 antibody|Cyclic AMP dependent transcription factor ATF 1 antibody|Cyclic AMP dependent transcription factor ATF1 antibody|Cyclic AMP-dependent transcription factor ATF-1 antibody|EWS AFT1 antibody|FUS ATF 1 antibody|FUS/ATF 1 antibody|Protein TREB36 antibody|RNA binding protein activating transcription factor 1 fusion protein antibody|TREB 36 antibody|TREB36 antibody|TREB36 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ATF1 antibody, ASA-B0163 Western blot
All lanes: Anti ATF1 (ASA-B0163) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted band size: 29KD
Observed band size: 29KD
All lanes: Anti ATF1 (ASA-B0163) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted band size: 29KD
Observed band size: 29KD
Anti-ATF1 antibody, ASA-B0163, IHC(P)
Human Intestinal Cancer Tissue
