B MyB Antibody

B MyB Antibody

$330.00

In stock

SKU: ASA-B0182 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
v-myb myeloblastosis viral oncogene homolog(avian)-like 2 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB(1-17aa MSRRTRCEDLDELHYQD), identical to the related mouse sequence and different from the related rat sequence by two amino acids.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 MYBL2

Protein

 Myb-related protein B

Uniprot ID

 P10244

Function

 Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

Tissue Specificity

  

Sub-cellular localization

 Nucleus.

Sequence Similarities

 Contains 3 HTH myb-type DNA-binding domains.

Aliases

 B-Myb antibody|BMyB antibody|MGC15600 antibody|MYB L2 antibody|Myb related protein B antibody|Myb-like protein 2 antibody|Myb-related protein B antibody|MybB antibody|MYBB_HUMAN antibody|MYBL 2 antibody|MYBL2 antibody|v myb avian myeloblastosis viral oncogene homolog like 2 antibody|v myb myeloblastosis viral oncogene homolog(avian) like 2 antibody|v myb myeloblastosis viral oncogene homolog like 2 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat Mouse AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat Mouse AssaySolutio’s IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, Rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-B MyB antibody, ASA-B0182, Western blotting
E.coli derived -recombinant Human MYBL2 (162aa tag+ M1-L245), 46.8KD
Lane 1: Recombinant Human MYBL2 Protein 10ng
Lane 2: Recombinant Human MYBL2 Protein 5ng
Lane 3: Recombinant Human MYBL2 Protein 2.5ng
 
Anti-B MyB antibody, ASA-B0182, IHC(P)
Rat Brain Tissue
 
Anti-B MyB antibody, ASA-B0182, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate
 

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