Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| BCL2-antagonist/killer 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human BAK1 (74-95aa, QVGRQLALIGDDINRRYDSEFQ), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
BAK1 |
Protein |
Bcl-2 homologous antagonist/killer |
Uniprot ID |
Q16611 |
Function |
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. |
Tissue Specificity |
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. |
Sub-cellular localization |
Mitochondrion membrane ; Single-pass membrane protein . |
Sequence Similarities |
Belongs to the Bcl-2 family. |
Aliases |
Apoptosis Regulator Bak antibody|BAK antibody|BAK like antibody|Bak NT antibody|BAK_HUMAN antibody|BAK1 antibody|Bcl 2 homologous antagonist/killer antibody|Bcl 2 like 7 protein antibody|Bcl-2 homologous antagonist/killer antibody|Bcl-2-like protein 7 antibody|BCL2 antagonist/killer 1 antibody|Bcl2 like 7 Protein antibody|Bcl2-L-7 antibody|BCL2L7 antibody|CDN1 antibody|Cell death inhibitor 1 antibody|MGC117255 antibody|MGC3887 antibody|NBak antibody|Pro apoptotic protein BAK antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat, Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BAK antibody, ASA-B0193, Western blotting
All lanes: Anti BAK (ASA-B0193) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted band size: 23KD
Observed band size: 23KD
All lanes: Anti BAK (ASA-B0193) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Predicted band size: 23KD
Observed band size: 23KD
Anti-BAK antibody, ASA-B0193, IHC(P)
Rat Lung Tissue
Rat Lung Tissue
