Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| branched chain amino-acid transaminase 2, mitochondrial | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT2 (377-392aa, KEIQYGIRAHEWMFPV). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
BCAT2 |
Protein |
Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT(m)) |
Uniprot ID |
O15382 |
Function |
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. |
Tissue Specificity |
Ubiquitous. |
Sub-cellular localization |
Isoform A: Mitochondrion. |
Sequence Similarities |
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. |
Aliases |
BCAM antibody|BCAT 2 antibody|BCAT(m) antibody|BCAT2 antibody|BCAT2_HUMAN antibody|BCATM antibody|BCT2 antibody|Branched chain aminotransferase 2, mitochondrial antibody|Branched-chain-amino-acid aminotransferase antibody|ECA40 antibody|mitochondrial antibody|Placental protein 18 antibody|PP18 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-BCAT2 antibody, ASA-B0201, Western blotting
All lanes: Anti BCAT2 (ASA-B0201) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted band size: 41KD
Observed band size: 41KD
All lanes: Anti BCAT2 (ASA-B0201) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted band size: 41KD
Observed band size: 41KD
Anti-BCAT2 antibody, ASA-B0201, IHC(P)
Human Colon Cancer Tissue
Human Colon Cancer Tissue
