Bcl-2 Antibody (monoclonal)

Bcl-2 Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0203 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
B-cell CLL/lymphoma 2 Monoclonal IgG Mouse Human IHC-P, IHC-F, ICC, WB Ascites

Immunogen
Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 BCL2

Protein

 Apoptosis regulator Bcl-2

Uniprot ID

 Human(P10415), Mouse(P10417), Rat(P49950)

Function

 Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Tissue Specificity

 Expressed in a variety of tissues.

Sub-cellular localization

 Mitochondrion outer membrane.

Sequence Similarities

 Belongs to the Bcl-2 family.

Aliases

 BCL2|PPP1R50|protein phosphatase 1, regulatory subunit 50|ONCOGENE B-CELL LEUKEMIA 2

Application Details

Application Concentration* Species Validated Using**
Western blot 1-2μg/ml Human AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.4-1μg/ml Human AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.4-1μg/ml Human AssaySolutio’s IHC/ICC Detection kit
Immunocytochemistry Suitable Human AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Bcl-2 antibody (monoclonal), ASA-B0203, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Small Intestine Tissue Lysate
Lane 4: Rat Liver Tissue Lysate

 
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